اثر فواصل آبیاری بر شاخص‌های رشدی، فیزیولوژیکی و بیوشیمیایی سرخارگل (Echinaceae purpurea (L.) Monch) تحت محلول‌پاشی اسیدهیومیک

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشگاه علوم کشاورزی و منابع طبیعی گرگان

2 عضو هیات علمی دانشگاه علوم کشاورزی و منابع طبیعی گرگان

3 معاون بخش تولیدات گیاهی جهاد کشاورزی گلستان

4 استادیار گروه شیمی دانشگاه گلستان

چکیده

سابقه و هدف: خشکی از مهمترین عوامل محدودکنندهرشد گیاهان در سرتاسر جهان و شایع‌ترین تنش محیطی است. باتوجه به خطر جدی خشکی و کمبود آب، استفاده از روش‌های مناسب جهت بهبود اثرات منفی ناشی خشکی موردتوجه گرفته است. اخیراً استفاده از انواع اسیدهای آلی برای بهبود کمی و کیفی محصولات زراعی و باغی رواج فراوان یافته‌است. مقادیر بسیار کم از اسیدهای آلی به‌ دلیل وجود ترکیبات هورمونی، اثرات قابل‌‌ملاحظه‌ای در بهبود خصوصیات فیزیکی، شیمیایی و زیستی خاک و افزایش تولید و بهبود کیفیت محصولات کشاورزی دارند. لذا در همین راستا آزمایشی با هدف بررسی اثر رژیم‌های آبیاری و محلول‌پاشی اسیدهیومیک بر برخی ویژگی‌های مورفوفیزیولوژیکی و بیوشیمیایی گیاه دارویی سرخارگل انجام شد.
مواد و روش‌ها: این آزمایش در سال زراعی 96-1395به‌صورت کرت‌های خرد شده در قالب طرح بلوک‌های کامل تصادفی در سه تکرار در مزرعه تحقیقاتی مؤسسه غیرانتفاعی بهاران گرگان به ‌اجرا درآمد. تیمارهای آزمایش در چهار سطح دورآبیاری (شامل سه روز به‌عنوان شاهد، شش، نه و دوازده روز یکبار آبیاری) و چهار سطح محلول‌پاشی اسیدهیومیک (شامل صفر، 150، 300 و 450 میلی‌گرم‌درلیتر) در کرت‌هایی با ابعاد یک در یک‌و‌نیم متر لحاظ گردید. صفات مورد‌ارزیابی عبارت از ارتفاع بوته، طول ریشه، وزن تازه و خشک بوته، وزن تازه و خشک ریشه، پرولین برگ، محتوای نسبی آب برگ، کارتنوئید، فعالیت آنتی‌اکسیدانی، فنل‌کل و قند محلول بودند.
یافته‌ها: نتایج حاکی از آن بود که افزایش فاصله آبیاری سبب کاهش معنی‌دار ارتفاع بوته، طول ریشه، وزن تر گیاه، وزن تر و خشک ریشه گردید. در حالی‌که بیشترین میزان وزن خشک گیاه (41/9 گرم در گیاه) و طول ریشه (10/40 سانتی‌متر) به‌ترتیب از شش و نه روز یکبار آبیاری مشاهده شد. همچنین اثر دور آبیاری در بالاترین سطح منجربه کاهش 88/29 درصدی محتوای نسبی آب برگ و افزایش 29/69 درصدی پرولین نسبت به شاهد گردید. میزان فعالیت آنتی‌اکسیدانی و قند محلول در اثر دور آبیاری افزایش یافت و در سطح سوم آبیاری (نه روز یکبار آبیاری) به‌ترتیب 32/79 و 21/50 میلی‌گرم در میلی‌لیتر به حداکثر مقدار خود رسید. تیمار اسیدهیومیک نیز بر تمامی صفات اندازه‌گیری شده مورد بررسی به‌جز کارتنوئید اثر معنی‌داری داشت و این تأثیر در 450 میلی‌گرم در لیتر به حداکثر خود رسید در شرایط نه روز یک‌بار آبیاری محلول‌پاشی با 450 میلی‌گرم در لیتر موجب افزایش 58/0 درصدی پرولین نسبت به عدم مصرف آن در این شرایط گردید. حداکثر مقدار فعالیت آنتی‌اکسیدانی (67/82 میلی‌گرم در میلی‌لیتر) از سطح سوم دور آبیاری و کاربرد 150 میلی‌گرم در لیتر اسیدهیومیک و بیشترین مقدار قند محلول (56/53 میلی‌گرم در گرم) از سطح سوم دور آبیاری و کاربرد 300 میلی‌گرم در لیتراسیدهیومیک مشاهده شد.
نتیجه‌گیری: نتایج این پژوهش نشان داد که افزایش فاصله آبیاری به‌طور معنی‌داری سبب کاهش صفاتی نظیر ارتفاع بوته، طول ریشه، وزن تازه و خشک بوته، وزن تازه و خشک ریشه و محتوای نسبی آب برگ، گردید. در مقابل میزان برخی صفات نظیر پرولین، فعالیت آنتی‌اکسیدانی،‌ فنل‌کل و قند محلول افزایش یافت. با این‌حال اسیدهیومیک از گیاهان سرخارگل در برابر تنش خشکی محافظت کرد و سبب کاهش خسارات ناشی از خشکی گردید. به‌طور کلی نتایج این آزمایش، کاربرد 300 میلی‌گرم در لیتر اسیدهیومیک در شرایط 9 روز یکبار آبیاری را به‌عنوان بهترین تیمار از لحاظ اقتصادی معرفی می‌کند، زیرا با مصرف کمتر آب و اسیدهیومیک، می‌توان به همان میزان عملکرد متابولیت‌ها که سطوح بالاتر این کاربردها دارند، دست یافت.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of irrigation intervals on growth, physiological and biochemical indices of coneflower (Echinaceae purpurea (L.) Monch) under humic acid foliar application

نویسندگان [English]

  • sarah khorasaninejad 2
  • morteza sadeghi 3
  • pouneh ebrahimi 4
1
2 Faculty member of Gorgan University of Agricultural Sciences and Natural Resources
3 jahad keshavarzi of golestan province
4 Assistant professor of Golestan University
چکیده [English]

Background: Drought is one of the most important factors limiting the growth of plants around the world and the most common environmental stress. Considering the serious risk of drought and water scarcity, the use of appropriate methods to improve the negative effects of drought has attracted special interest. Recently, different types of organic acids have been widely used to improve the quality and quantity of crops and horticultural products. Due to the hormonal compounds, extremely low amounts of organic acids have significant effects on the improvement of physical, chemical and biological properties of the soil and the production and quality of agricultural products. In this regard, an experiment was conducted to investigate the effect of irrigation intervals and the foliar application of humic acid on some morphophysiological and biochemical characteristics of coneflower.
Materials and Methods: This experiment was conducted in the 2016-17 crop year with split plot arrangement in a randomized complete block design with three replications at the research farm of the Baharan non-profit organization in Gorgan. The treatments were included four levels of irrigation intervals (including the irrigation every one, three, six and nine days) and four levels of humic acid foliar application (including 450, 300, 150 and 0 mg/L). The evaluated traits included fresh and dry weight of plant, fresh and dry weight of root, proline, relative water content (RWC) of leaves, antioxidant activity, total phenol and soluble sugar. After planting, growing and harvesting, the measurement of proline growth performance was done by the Bates method, total phenol by the Folin-Ciocalteu method, carotenoid by the Barnes method, and soluble sugar by the Omokolo method.
Results: The results showed that the increased levels of irrigation intervals caused a significant decrease in plant height, root length, fresh weight of plant, and fresh and dry weight of root, while the highest dry weight of the plant (9.41 g per plant) and root length (10.40 cm) was observed from the second and third levels of irrigation interval, respectively. Also, the effect of irrigation interval at the highest level resulted in 29.88% reduction in the relative water content of leaf and 69.29% increase in proline compared with the control. The level of antioxidant activity and soluble sugar was increased with the irrigation intervals, and at the third level of irrigation (irrigation every six days), it was at most 71.39 and 43.56 mg/mL, respectively. Humic acid treatment had a significant effect on all of the measured traits except carotenoid, and the effect reached to the maximum level at 450 mg/L. In the conditions of irrigation every nine days, the foliar application with 450 mg/L resulted in a 0.58% increase in proline compared with not to use it in this condition. The maximum level of antioxidant activity (82.67 mg/mL) from the third level of irrigation interval and application of 150 mg/L of humic acid and the highest level of soluble sugar (53.56 mg/g) from the third level of irrigation interval and application 300 mg/L of humic acid was observed.
Conclusion: The results of this study showed that increasing the irrigation interval significantly reduced the morphological traits. In contrast, some traits such as proline, antioxidant activity, total phenol, and soluble sugar were increased. However, humic acid protects the coneflower plants from the drought stress and reduces the drought damage. In general, the results of this study introduced the foliar application of 300 mg/L of humic and every 9 days irrigiration as the best treatment, economically. Because with less water and humic acid can be reached to the same methabolites yield that higher levels of them.

کلیدواژه‌ها [English]

  • Antioxidant activity
  • Dehydration stress
  • Organic fertilizer
  • Proline
  • Total phenol

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مجله تولید گیاهان زراعی
دوره 12، شماره 3
آذر 1398
صفحه 101-120

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