Evaluation of the effect of different urea fertilizer levels on yield stability of two rainfed lentil cultivars in the Maragheh region

Document Type : Complete scientific research article

Authors

1 Yaser Azimzadeh Dryland Agricultural Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Maragheh, Iran

2 Dryland Agricultural Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Maragheh, Iran

10.22069/ejcp.2026.24127.2714

Abstract

Background and objectives: Lentil plays a crucial role in food security, while nitrogen fertilization is key to achieving stable yields in dryland cropping systems. Determining the optimal nitrogen rate for sustainable lentil production under climate variability in semi-arid regions is therefore essential. Although lentil is considered a low-nitrogen-input crop due to its ability to form symbiotic relationships with nitrogen-fixing bacteria, the application of starter nitrogen fertilizer becomes critical in soils with low organic matter content – a common characteristic of most Iranian drylands – to ensure proper crop establishment and yield potential realization. However, the response of lentil to nitrogen under dryland conditions is significantly influenced by annual rainfall fluctuations, and multi-year studies on this topic remain limited in Iran. Accordingly, this study was designed and conducted to evaluate the effects of different urea fertilizer levels on yield stability of two rainfed lentil cultivars (Sana and Bilehsavar) in response to annual climatic variability in the Maragheh region.
Materials and methods: The experiment was conducted as a split-plot arrangement within a randomized complete block design with three replications at the Maragheh Dryland Agricultural Research Station over two growing seasons (2023-2025). Main plots were assigned to lentil cultivars (Sana and Bilehsavar), while sub-plots consisted of nitrogen levels (0, 25, 50, 75, and 100 kg urea ha⁻¹). Following field preparation using minimum tillage with a composite tiller, spring planting was carried out in 10 × 30 m plots using a three-unit ASKE seeder at a planting depth of 5 cm with 17.5 cm row spacing. Urea fertilizer was applied simultaneously with planting through band placement at 5-6 cm depth beneath the seed.
Results: Combined analysis of variance revealed significant (p < 0.01) effects of year, cultivar, and urea levels on all studied traits except harvest index. Significant year × cultivar interactions were observed for grain yield and harvest index, while year × urea interactions affected plant height and biological yield. During the first year with favorable precipitation (381 mm), maximum grain yields of 766 kg ha⁻¹ for Sana and 603 kg ha⁻¹ for Bilehsavar were recorded. However, under reduced rainfall (280 mm) in the second year, yields declined to 622 and 520 kg ha⁻¹ for Sana and Bilehsavar, respectively. Quadratic regression models indicated optimal urea rates ranging between 45-50 kg ha⁻¹ for Sana and 47 kg ha⁻¹ for Bilehsavar to achieve maximum grain yield. Notably, Sana demonstrated significantly higher rain water productivity (1.93 kg ha⁻¹ mm⁻¹) compared to Bilehsavar (1.59 kg ha⁻¹ mm⁻¹).
Conclusion: In summary, the Sana cultivar demonstrated superior adaptation to the variable climatic conditions of the Maragheh region, exhibiting higher yield stability, improved harvest index (44%), and enhanced rain water productivity (1.93 kg ha⁻¹ mm⁻¹). Based on the research findings, the application of 45-50 kg urea ha⁻¹ for the Sana cultivar is recommended as an optimal strategy for achieving sustainable and economic yields in dryland lentil production systems.

Keywords

Main Subjects


  1. . Dhaliwal, S.S., Sharma, V., Shukla, A.K., Kaur, J., Verma, V., Singh, P., & Hossain, A. (2021). Enrichment of zinc and iron micronutrients in lentil (Lens culinaris Medik.) through biofortification. Molecules, 26(24), 7671.‏

    1. Sabaghpour, S.H., Shahbazi, H., & Rezakhanlou, R. (2019). The effect of using biological and chemical nitrogen fertilizers on yield and yield components in lentil cultivars. Iranian Journal Pulses Research, 10(1), 40-51. (In Persian with English Abstract)
    2. Bannayan Aval, M., Yaghoubi, F., Rashidi, Z., & Bardehji, S. (2018). Effect of different nitrogen levels on phenology, growth indices and yield of two lentil cultivars under rainfed conditions in Mashhad. Iranian Journal of Field Crops Research, 15(4), 939-956. (In Persian with English Abstract)
    3. Baber, K., Jones, C., Miller, P., Lamb, P., & Atencio, S. (2023). Lentil nitrogen fixation response to fertilizer and inoculant in the northern Great Plains. Agronomy Journal, 115(5), 2614-2630.‏
    4. Mesgaran, M.B., Madani, K., Hashemi, H., & Azadi, P. (2017). Iran’s land suitability for agriculture. Scientific reports, 7(1), 7670.‏
    5. Turay, K.K., Andrews, M., & McKenzie, B.A. (1991). Effects of starter nitrogen on early growth and nodulation of lentil (Lens culinaris Medik.). In Proceedings of the agronomy society of New Zealand (21, pp. 61-65).‏
    6. Seyed Sharif, R., & Seyed Sharifi, R. (2020). Effects of starter nitrogen, methanol and bio fertilizers application on yield, nodulation and grain filling period of rainfed lentil. Journal of Crops Improvement, 22(3), 445-460.‏ (In Persian with English Abstract)
    7. Walley, F.L., Boahen, S.G., Hnatowich, K., & Stevenson, C. (2005). Nitrogen and phosphorus fertility management for desi and kabuli chickpea. Canadian Journal of Plant Science, 85, 73-79.
    8. Rahman, M.M., Ashutosh Sarker, A.S., Shiv Kumar, S.K., Asghar Ali, A.A., Yadav, N.K., & Rahman, M.L. (2009). Breeding for short season environments. In The lentil: Botany, production and uses (pp. 121-136). Wallingford UK: CABI.‏
    9. Nadeem, M., Li, J., Yahya, M., Wang, M., Ali, A., Cheng, A., & Ma, C. (2019). Grain legumes and fear of salt stress: Focus on mechanisms and management strategies. International Journal of Molecular Sciences, 20(4), 799.‏
    10. Sun, X., Miao, Q., Gu, Y., Yang, L., & Wang, P. (2025). Research on the physiological mechanisms of nitrogen in alleviating plant drought tolerance. Plants, 14(18), 2928.‏
    11. Zhou, M., Li, Y., Yao, X.L., Zhang, J., Liu, S., Cao, H.R., & Chen, Z.C. (2024). Inorganic nitrogen inhibits symbiotic nitrogen fixation through blocking NRAMP2-mediated iron delivery in soybean nodules. Nature Communications, 15(1), 8946.‏
    12. Bannayan, M., Yaghoubi, F., Rashidi, Z., & Bardehji, S. (2019). Effect of different nitrogen levels on yield components, yield and nitrogen use efficiency of two lentil cultivars in rainfed conditions. Iranian Journal of Pulses Research10(1), 155-170.‏ (In Persian with English Abstract)
    13. Dane, J.H., & Topp, C.G. (2020). Methods of Soil Analysis, Part 4: Physical Methods. John Wiley & Sons.‏
    14. Sparks, D.L., Page, A.L., Helmke, P.A., & Loeppert, R.H. (2020). Methods of Soil Analysis, part 3: Chemical methods. John Wiley & Sons.‏
    15. Azimzadeh, Y., Mohammadzadeh, A., Hasanian Khoshroo, H., & Ghahramanian, G. (2025). Investigating the response of rainfed chickpea to different nitrogen fertilizer levels in cold regions. Iranian Journal Pulses Research, 16(1), 177-191. (In Persian with English Abstract)
    16. Rockström, J., Karlberg, L., Wani, S.P., Barron, J., Hatibu, N., Oweis, T., & Qiang, Z. (2010). Managing water in rainfed agriculture—The need for a paradigm shift. Agricultural Water Management, 97(4), 543-550.‏
    17. Dhakal, C., & Lange, K. (2021). Crop yield response functions in nutrient application: A review. Agronomy Journal113(6), 5222-5234.‏
    18. Shilpha, J., Song, J., & Jeong, B.R. (2023). Ammonium phytotoxicity and tolerance: An insight into ammonium nutrition to improve crop productivity. Agronomy, 13(6), 1487.‏
    19. Aghabeygi, M., & Dönmez, C. (2024). Estimating yield response functions to nitrogen for annual crops in Iran. Agronomy Journal, 14(3), 436.‏
    20. Gan, Y., Stulen, I., van Keulen, H., & Kuiper, P.J. (2003). Effect of N fertilizer top-dressing at various reproductive stages on growth, N2 fixation and yield of three soybean (Glycine max (L.) Merr.) genotypes. Field Crops Research, 80(2), 147-155.‏
    21. Humayun, K.M., Pronabananda, D., Monirul, I.M., Belal, H.M., Mamun, A.N.K., & Roland, V.R. (2019). Effect of different doses of nitrogen on nitrogen fixation and yield of lentil using tracer technique. GSC Biological and Pharmaceutical Sciences, 6, 69-75.‏
    22. Kumar, S., Sharma, S.K., Dhaka, A.K., Bedwal, S., Sheoran, S., Meena, R.S., & Hossain, A. (2023). Efficient nutrient management for enhancing crop productivity, quality and nutrient dynamics in lentil (Lens culinaris Medik.) in the semi-arid region of northern India. Plos one, 18(2), e0280636.‏
    23. Huang, J., Keshavarz Afshar, R., & Chen, C. (2016). Lentil response to nitrogen application and rhizobia inoculation. Communications in soil science and plant analysis, 47(21), 2458-2464.‏
    24. Gutierrez, A.F., Rodriguez-Torres, A.F., & Gutierrez-Gonzalez, J.J. (2025). Accurate screening for drought tolerance in lentils (Lens culinaris Medik). Plant Stress, 100994.‏
    25. El Haddad, N., Choukri, H., Ghanem, M.E., Smouni, A., Mentag, R., Rajendran, K., & Kumar, S. (2021). High-temperature and drought stress effects on growth, yield and nutritional quality with transpiration response to vapor pressure deficit in lentil. Plants, 11(1), 95.‏