Investigation of fluorescence chlorophyll sensitivity, chlorophyll index, rate of Chlorophyll (a, b), nitrogen concentration and nitrogen nutrition index under under nitrogen and phosphorus nutrition in wheat

Document Type : Research Paper



In scale universal after drought, nitrogen is the most important factor limiting. After nitrogen, phosphorus is the most nutritious element for plant. Between nitrogen and phosphorus is a positive reaction and absorption nitrogen increases absorption phosphorus by plant. The Studies very few is performed in order to study fluorescence chlorophyll plant in nutritious shortage stress conditions. Reduction access to nitrogen decreases quantum yield in transfer electron (photo system II) and its maximum efficiency. Also shortage of nitrogen destroys photo system II and decreases fluorescence variable (Fv). Quantum yield increases consequently interaction effects nitrogen and phosphorus. In view of the fact that measurement concentration nitrogen with methods like measuring Nitrogen Nutrition Index (NNI) is very costly, take up time and need to expensive equipment, so measurement chlorophyll index by hand chlorophyll meter and transportable is very comfortable. Also consumption of nitrogen is improved pigments, as rate of chlorophyll (a, b) increases by using nitrogen. this research in order to study relations between concentration nitrogen, chlorophyll (a, b), chlorophyll index and nitrogen nutrition index (NNI) and study parameters fluorescence chlorophyll and rate of chlorophyll (a and b) under effect of different treatment fertilizer of nitrogen and phosphorus in wheat was performed. Afield experiment was conducted in a factorial arrangement using randomized complete block design with 4 replications in Aliabadkatool city of Golestan province in 2014-2015 and 2015-2016 growing seasons. The experimental factors were three net nitrogen fertilizer (N, 0, 163 and 326 kg ha-1 and five net phosphorus fertilizer (P, 0, 98, 196, 294 and392 kg ha-1.) In this experiment some traits such as chlorophyll index, nitrogen nutrition index, fluorescence chlorophyll parameters, rate of chlorophyll (a, b), nitrogen concentration, grain protein and grain yield were measured. The study of scattering diagram of data be showed, traits of chlorophyll index, nitrogen nutrition index, quantum yield, chlorophyll concentration (a, b), nitrogen concentration in plan and yield protein follow from simple linear regression model, as in each level nitrogen, with increase rate of fertilizer phosphorus, traits under study were prospered from increasing trend.Concentration of nitrogen and nitrogen nutrition index was under effect interaction year and nitrogen. Fluorescence chlorophyll parameters including minimum fluorescence, maximum fluorescence (Fo, Fm) against other traits were showed decreasing trend, That connected to increase quantum yield consequently increase in consumed fertilizer. Correlation of significant and positive between chlorophyll index, nitrogen nutrition index, nitrogen concentration, chlorophyll was observed. The maximum yield achieved at combination of fertilizer N326P392 and N326P294that point of view statically was in one of surface significantly. The results this experiment showed that very strong relation is between chlorophyll index, chlorophyll concentration, Nitrogen nutrition index and nitrogen available to plant, as with increase available nitrogen and phosphorus rate, quantum yield and grain yield increased grain yield, so can be used from chlorophyll meter set for assessment fertilizer in plant replacing methods of expensive and take up tim like nitrogen nutrition index. In management fertilizer in addition on nitrogen, phosphorus must be considered too.


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