Effects of mycorrhiza and superabsorbent on root morphological characteristics and yield of chickpea (Cicer arietinum L.) under rain-fed conditions

Document Type : Research Paper

Author

Abstract

Background and aim: inclusion of legumes in rotation to reduce chemical fertilizers has been of interest. The use of biological resources instead of chemicals can play an important role in maintaining fertility and soil biological activities; also increased the quality of agricultural products and ecosystem health. Applying mycorrhiza and superabsorbent as factors of preserving water and supplying nutrients for plants are important in dry farming.
Materials and methods: The experiment was conducted in Lorestan province (Noor Abad city) as a split-factorial arrangement based on a randomized complete block design (RCBD) with three replications. Two different chickpea cultivars (‘Arman’ and ‘Azad’) were used as the main plot and superabsorbent polymer at three levels (0, 100 and 200 kg ha-1) and mycorrhiza at two levels (0 and 200 kg ha-1) were served as the sub-plots.
Finding: Cultivars were not different according to considered traits. Using superabsorbent (200 kg/ha) increased biological yield (5.9%), grain yield (9.4%), root length (18.5%), number of nodules (19.7%) and root volume (21.9%). Results indicated that mycorrhiza application improved biological yield (4.7%), grain yield (4%), root length (12.8%), root dry weight (8.3%), and root volume (11.7%). The interaction of superabsorbent and mycorrhiza was statistically significant on biological yield, grain yield, root length, and the number of root nodules of chickpea.
Results: applying superabsorbent and mycorrhiza increased root traits and grain yield, however, when they were used together, their positive effects on the yield and root traits were more than they were applied separately. Regarding to the water resources shortage in the country, simultaneous using of mycorrhiza and superabsorbent; in addition to improving chickpea yield under rain-fed conditions, can provide bases for organic agriculture, system sustainability, finally more environment health.
Finding: Cultivars were not different according to considered traits. Using superabsorbent (200 kg/ha) increased biological yield (5.9%), grain yield (9.4%), root length (18.5%), number of nodules (19.7%) and root volume (21.9%). Results indicated that mycorrhiza application improved biological yield (4.7%), grain yield (4%), root length (12.8%), root dry weight (8.3%), and root volume (11.7%). The interaction of superabsorbent and mycorrhiza was statistically significant on biological yield, grain yield, root length, and the number of root nodules of chickpea.
Results: applying superabsorbent and mycorrhiza increased root traits and grain yield, however, when they were used together, their positive effects on the yield and root traits were more than they were applied separately. Regarding to the water resources shortage in the country, simultaneous using of mycorrhiza and superabsorbent; in addition to improving chickpea yield under rain-fed conditions, can provide bases for organic agriculture, system sustainability, finally more environment health.

Keywords

Main Subjects


1. Abbott, L.K., and Murphy, D.V. 2007. Soil Biological Fertility: A key to sustainable land
use in agriculture. Kluwer Academic Publishers. 264 pages.
2. Aerts, R., and Chapin, F.S. 1999. The mineral nutrition of wild plants revisited: A reevaluation
of processes and patterns. Adv. Ecol. Res. 62: 26-34.
3. Alimadadi, A., Jahansouz, M.R., Besharati, H., Tavakkol-Afshari, R., and Tavakkoli, M.
2010. Effect phosphate solubilizing micro-organisms, mycorrhiza and seed priming on the
nodulation in chickpea crop (Cicer arietinum L.). Iranian J. Soil Res. (Soil Science and
Water) 24(1): 43-53. (In Persian)
4. Alizadeh, A. 1999. Plants and Soil-Water Relationships. University of Mashhad, 353p. (In
Persian)
5. Alloush, G.A., Zeto, S.K., and Clark, R.B. 2000. Phosphorus source, organic matter, and
arbuscular mycorrhiza effects on growth and mineral acquisition of chickpea grown in acidic
soil. Plant Nut. 23: 1351-1369.
6. Ardakani, M.R., Mazaheri, D., Majd, F., and Normohamadi, G. 2000. Study mycorrhiza and
Streptomyces efficiency and different levels of phosphorus, on grain yield and some
characters of Wheat. Iran. J. Crop Si. 22: 2-17. (In Persian)
7. Asgharipoor, M.R., and Rafiei, M. 2010. Effect of drought stress on different morphological
characteristics of root and root: shoot ratio on mungbean genotypes. Pp: 2814. In:
Proceedings of the 11th Iranian Crop Sciences Congress, Shahid Beheshti University,
Tehran, Iran. (In Persian)
8. Atiyeh, R.M., Arancon, N.Q., Edwards, C.A., and Metzger, J.D. 2002. The influence of
earthworm-processed pig manure on the growth and productivity of marigolds. Bioresource
Technol. 81: 103-108.
9. Auge, R.M. 2001. Water relations, drought and vesicular-arbuscular mycorrhizal symbiosis.
Mycorrhiza, 11: 3-42.
10. Azarnia, M., and Eisvand, H.R. 2014. Effects of hydro and hormonal priming on yield and
yield components of chickpea (Cicer arietinum L.) in irrigated and rain-fed conditions.
EJCP., 6(4): 1-18. (in Persian)
11. Bianciotto, V., Andreotti, S., Balestrini, R., Bonfante, P., and Perotto, S. 2001. Extracellular
polysaccharides are involved in the attachment of Azospirillum brasilense and Rhizobium
leguminosarum to arbuscular mycorrhizal structures. Eur. J. Histochem. 45: 39-49.
12. Bowen, G.D., and Rovira, A.D. 1999. The rhizosphere and its management to improve plant
growth. Adv. Agron. 66: 1-102.
13. Degiorgi, C.F., Pizarro, R.A., Smolko, E.E., Lora, S., and Carenza, M. 2002. Hydrogels for
immobilization of bacteria used in treatment of metal-contaminated wastes. Radiat. Physics
Chem. 63: 109- 113.
14. Egamberdiyeva, D. 2007. The effect of plant growth promoting bacteria on growth and
nutrient uptake of maize in two different soils. Appl. Soil Ecol. 36: 184-189.
15. Eneji, A.E., Islam, R., An, P., and Amalu, U.C. 2013. Nitrate retention and physiological
adjustment of maize to soil amendment with superabsorbent polymers. Cleaner Prod. 52:
474-480.
16. Eubeler, J.P., Bernhard, M., and Knepper, T.P. 2010. Environmental biodegradation of
synthetic polymers ll. Biodegradation of different polymer groups. TrAC Trends Anal.
Chem. 29: 84-100.
17. Ganjeali, A., Kaffi, M., and Sabet Teimouri, M. 2010. Variations of root and shoot
physiological indices in chickpea (Cicer arietinum L.) in response to drought stress. Env.
Stresses Crop Sci. 3: 35-45. (in Persian)
18. Gehan, G., Mostafa, A., and Abo-Baker, A.A. 2010. Effect of bio-and chemical fertilization
on growth of sunflower (Helianthus annuus L.) at south valley area. Asian J. Crop Sci. 2:
137-146.
19. Grant, C.A., Peterson, G.A., and Campbell, C.A. 2002. Nutrient consideration for diversified
cropping systems in the northern great plains. Agron. J. 94: 186-198.
20. Grossnickle, S.C. 2005. Importance of root growth in overcoming planting stress. New
Forests. 30: 273-294.
21. Gupta, M.L., Prasad, A., Ram, M., and Kumar, S. 2002. Effect of the vesicular-arbuscular
mycorrhizal (VAM) fungus Glomus fasciculatum on the essential oil yield related characters
and nutrient acquisition in the crops of different cultivars of menthol mint (Menthe arvensis)
under field conditions. Bioresource Technol. 81: 77-79.
22. Islam, M.R., Hu, Y., Mao, S., Mao, J., Eneji, A.E., and Xue, X. 2011. Effectiveness of a
water-saving super-absorbent polymer in soil water conservation for corn (Zea mays L.)
based on eco-physiological parameters. Sci. Food Agric. 91: 1998–2005.
23. Kapoor, R., Giri, B., and Mukerji, K.G. 2004. Improved growth and essential oil yield and
quality in Foenciulum vulgare mill on mycorrhizal inoculation supplemented with Pfertilizer.
Bioresource Technol. 93: 307-311.
24. Khadem, S.A., Ghalavio, M., Ramroodi, S.R., Mousavi, M.J., and Rezvani-Moghadam, P.
2011. Effect of animal manure and superabsorbent polymer on yield and yield components
on corn (Zea mays L.). Iran. J. Crop Sci. 1: 115-123. (in Persian)
25. Khalvati, M.A., Hu, Y., Mozafar, A., and Schmidhalter, U. 2005. Quantification of water
uptake by arbuscular mycorrhizal hyphae and its significance for leaf growth, water
relations, and gas exchange of barley subjected to drought stress. Plant Biol. 7: 706-712.
26. Li, K.Y., De Jong, R., Coe, M.T., and Ramankutty, N. 2006. Root-water-uptake based upon
a new water stress reduction and an asymptotic root distribution function. Earth Interac. 10:
1-22.
27. Michele, A., Douglas, T., and Frank, A. 2009. The effects of clipping and soil moisture on
leaf and root morphology and root respiration in two temperate and two tropical grasses.
Plant Ecol. 200: 205-215.
28. Moradi, S., Sheikhi, J., Zarei, M. 2013. Effects of arbuscular mycorrhizal fungi and
rhizobium on shoot and root growth of chickpea in a calcareous soil. Int. J. Agric. 3: 381-
385.
29. Mosse, B. 1986. Mycorrhiza in a sustainable agriculture. Biol. Agri. Horti. 3: 191-209.
30. Nazarli, H., Zardashti, M.R., Darvishzadeh, R., and Najafi, S. 2010. The effect of water
stress and polymer on water use efficiency, yield and several morphological traits of
sunflower under greenhouse condition. Not. Sci. Biol. 2: 53-58.
31. Pouresmaeil, P., Habibi, D., Tavasoli, A., Zahedi, H., Touhidi moghadam, H.R. 2010. The
effect of water super absorbent polymer on agronomic and physiological characters of red
bean varieties under drought stress in the greenhouse condition. Plant Eco. 21: 75-91.
32. Ratti, N., Kumar, S., Verma, H.N., and Gautam, S.P. 2001. Improvement in bioavailability
of tricalcium phosphate to cymbopogon martinii var. motia by rhizobacteria, AMF and
azospirillum inoculation. Microbiol. Res. 156(2): 145-149.
33. Sepaskhah, A.R., and Bazrafshan-Jahromi, A.R. 2006. Controlling runoff and erosion in
sloping land with polyacrylamide under a rainfall simulator. Biosystems Eng. 93: 469-474.
34. Sing, G., Sekhon, H.S., and Kolar, J.S. 2005. Pulses. Agrotech Publishing Academy.
Udaipur, India. 329p.
35. Singh, S., and Kapoor, K.K. 1998. Effects of inoculation of phosphate-solubilizing
microorganisms and an arbuscular mycorrhizal fungus on mungbean grown under natural
soil conditions. Mycorrhiza, 7: 249-253.
36. Solaiman, A.R.M., Rabbani, M.G., and Molla, M.N. 2005. Effects of inoculation of
rhizobium and arbuscular mycorrhiza, poultry litter, nitrogen, and phosphorus on growth and
yield in chickpea. Korean J. Crop Sci. 50: 256-261.
37. Toussaint, J.P., Smith, F.A., and Smith, S.E. 2007. Arbuscular mycorrhizal fungi can induce
the production of phytochemicals in sweet basil irrespective of phosphorus nutrition.
Mycorrhiza, 17: 291-297.
38. Vamerali, T., Saccomani, M., Bona, S., Mosca, G., Guarise, M., and Ganis, A. 2003. A
comparison of root characteristics in relation to nutrient and water stress in two maiz
hybrids. Plant Soil. 225: 157-167.
39. Zahir, Z.A., Arshad, M., and Frankenberger Jr, W.T. 2004. Plant growth promoting
Rhizobacteria: applications and perspectives in agriculture. Adv. Agron. 81: 97-168.
40. Zaidi, A., and Khan, M.S. 2006. Co-inoculation effects of phosphate solubilizing
microorganisms and Glomus fasciculatum on green gram-bradyrhizobium symbiosis. Tur. J.
Agri. Fores. 30: 223-230.
41. Zhong, K., Zheng, X.L., Mao, X.Y., Lin, Z.T., and Jiang, G.B. 2012. Sugarcane bagasse
derivative-based superabsorbent containing phosphate rock with water-fertilizer integration.
Carbohydrate Pol. 90: 820-826.