Salinity is one of the major problems in the world's vast areas of land are also faced with this problem. Endophytes are microorganisms that use of the soil microorganisms, the reduction of environmental stresses such as salinity, has become a global solution. Therefore, to investigate the effects of inoculation of fungal endophytes Piriformospora indica and bacteria symbiosis hay Azospirillum Sppon growth and characterization of physiological forage alfalfa under salt stress experiment in a factorial block design a completely randomized design with three replications in the greenhouse, Agricultural Research Ilam University was conducted. The treatments included two levels of application of fungal endophytes (fungi P.indica bacteria Azospirillum Spp (inoculated and non-inoculated), inoculated with fungus and bacteria and integrated control of soil salinity levels (0, 2, 4 g cholorid sodium/ kg soil), respectively. Positive and statistically significant effect on growth P.indica fungi, fresh and dry weight of forage, photosynthesis pigment, proline and nutrient uptake under saline conditions there, so that fungi use to reduce the adverse effects of salinity by reducing Toxic absorption of sodium and chloride and increased chlorophyll content improved growth of alfalfa hay. Haywere inoculated with bacteria from the proline and chlorophyll was higher. Inoculated with Rhizobium bacteria more favorable effect on chlorophyll content, proline and nutrient found. The results showed that the salinity conditions of thismicroorganisms in order to increase the quality and quantity of forage plants used for hay.