Effect of seed priming on antioxidant enzymes and lipids peroxidation of cell membrane in Black cumin (Nigella sativa) seedling under salinity and drought stress

Effect of seed priming on antioxidant enzymes and lipids peroxidation of cell membrane in Black cumin (Nigella sativa) seedling under salinity and drought stress

Document Type : Research Paper

Abstract

This study was aimed to assess of priming effects on soluble proteins and antioxidant enzymes of as a Nigella Sativa medicinal plant under different levels of salinity and drought. Two greenhouse factorial experiments with three replicated Randomized Complete Block Design were conducted.Treatments included five levels of priming (potassium nitrate 1% and salicylic acid 0.2 mM in 6 hours, potassium nitrate 3% and salicylic acid 0.5 mM in 12 hours and hydropriming in 24 hours) as main factor and four salinity levels (Control, 75, 125 and 175 mM NaCl) and three levels of drought by osmotic potential of Control, -3 bar and -6 bar by polyethylene glycol₆₀₀₀ as the second factor in each experiment. Results showed that the interaction of salinity and drought stress on seed priming were significant for all traits. Soluble protein concentration decreased along with increasing drought and salinity stress and antioxidant enzymes activity, (which proline plays a protective effect against stress), and MDA were increased. But, using priming, the antioxidant enzymes activity and proline content were increased while MDA was decreased. In this study, plants treated with integrated salicylic acid, potassium nitrate and distilled water had usually higher antioxidant activity (catalase, peroxidase and polyphenol oxidase) and soluble proteins in comparison with untreated seedlings.

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