Investigation of the Gelling agent, carbohydrate source and culture container on potato micropropagation (Solanum tuberosum L.)

Document Type : Research Paper

Abstract

Investigation of the effects of gelling agents, carbohydrate sources and culture containersonmicropropagation of three potato (Solanumtuberosum L.)cultivars
Zahra Movahedi1, Ahmad Moieni2*
Abstract
Background and objectives:Potato (Solanumtuberosum L.) is the fourth most important food crop in the world. Potato micropropagation can be affected by some factors including the type of culture container, carbohydrate source and gelling agent.
Materials and methods:In this study, the effects of above-mentioned different factors were investigated on the micropropagation of three potato cultivars (Agria, Marfona and Savalan) using MS medium. The effects of two type gelling agents (Agar Agar (7 gl-1)and Phytagel (3.5 gl-1) ), three carbohydrate sources (grade sucrose, table sugar, brown sugar (30 gl-1) ) and different culture containers (test tube, small glass bottle (110 × 60 mm), large glassbottle (150 × 95 mm) and polypropylenecontainer (90 × 90 ×110 mm) ) were investigated in the separated experiments. Each experiment was performed according to a factorial experiment based on completely randomized design (CRD) with 10 replications. After 30 days, different traits including shoot length per plantlet, mean of root length per plantlet, number of roots per plantlet, leaf size per plantlet, number of nodes per plantlet and mean of minituber weight per plantletwere measured.The regenerated plants were acclimatized and cultured in the field conditions for minituber production.
Results:The results indicated that the medium containing agar-agar as gelling agent had the highest shoot length per plantlet (5.16 cm) and mean of root length per plantlet (6.06 cm). The highest number of roots per plantlet (4.7) has been obtained by cultivars Marfona and Savalan. Cultivar Marfona also produced the maximum number of nodes per plantlet (9.2). Investigation of the carbohydrate source showed that the brown sugar has improved the most studied traits and the highest shoot length per plantlet (7.07 cm)and mean of root length per plantlet (6.61) have been obtained by the use of brown sugar. Also, the use of polypropylenecontainer (Vitro vent plant tissue container) improved the different studied characters including shoot length per plantlet(17.96 cm) andmean of root length per plantlet (11.73 cm).
Conclusion:In general, the use of agar–agar, brown sugar and polypropylene container can enhance the micropropagation rate and decrease the production input costs.Polypropylenecontainer has the unique capacity of continuous gas-exchange between the inner volume of the container and the outside environment. There is a continuous supply of fresh air in the container and no accumulation of volatile compounds.
Key words: Potato, Micropropagation, Gelling agent, Carbohydrate source, Culture container

Keywords

Main Subjects


1. Bajaj, Y.P.S. 1986. Biotechnology in agriculture and forestry. 2: Crops. Springer Verlag,
Berlin: 1-608.
2. Bowen, W.T. 2003. Water Productivity and Potato Cultivation. In: J.W. Kijne, R. Barker and
D. Molden (eds). Water Productivity in Agriculture: Limits and Opportunities for
Improvement. CAB International 2003. Pp: 229-238.
3. Corder, W.G., and Foreman, D.I. 2009. Nonparametric Statistics for Non-Statisticians: A
step-by-step Approach.Wiley. 264p.
4. Demo, P., Kuria, P., Nyende, A.B., and Kahangi, E.M. 2008. Table sugar as an alternative
low cost medium component for in vitro micro-propagation of potato (Solanum tuberosum
L.). Afr. J. Biotechnol, 7(15): 2578-2584.
5. Edwin, F.G., Hall, M.A., and De Klerk, G.J. 2008. Plant Propagation by Tissue Culture.
Spinger, Dordrecht, the Netherlands, 479p.
6. Islam, M.D., Dembele, D.P., and Keller, E.RJ. 2005. Influence of explants, temperature and
different culture vessels on in vitro culture for germplasm maintenance of four mint
accessions. Plant. Cell. Tiss. Org. 81: 123-130.
7. Jain, N., and Babbar, S.H. 2002. Gum katira- a cheap gelling agent for plant tissue culture
media. Plant. Cell. Tiss. Org. 71: 223-229.
8. Kacar, Y.A., Biçen, B., Varol, I., Mendi, Y.Y., Serçe, S., and Cetiner, S. 2010. Gelling
agents and culture vessels affect in vitro multiplication of banana plantlets. G.M.R, 9(1):
416-424.
9. Kornova, K., and Popov, S. 2007. Effect of the in vitro container type on growth
characteristics of the microplants in in vitro propagation of GF 677. I Balkan Symp Fruit
Grow. 825: 277–282.
10. Korosteleva, O. 2013. Nonparametric Methods in Statistics with SAS Applications.
Chapman and Hall/CRC. 195p.
11. Krikorian, A.D. 1995. Hormones in tissue culture and micropropagation. In Davies, P.J.
(ed.). Plant hormones: Physiology, Biochemistry and Molecular Biology. Kluwer, Dordrecht,
pp. 774-796.
12. Nestakova, M., Havrlentova, M., and Farago, J. 2000. Effect of gelling agents on in vitro
multiplication of two ornamental plants. Biologica Bratislava, 55(4): 409– 411.
13. Rahimian, N., Moieni, A., and Shams bakhsh, M. 2013. Investigation of some virus –
elimination methods and their effects on minituber yield in potato (Solanum tuberosum L.).
Iran. J. Field Crop Sci., 44: 623- 634. (In Persian)
14. Ramesh, Y., Ramanujam, V., and Ramassamy, V. 2014. Effect of gelling agents and Carbon
sources in in vitro multiplication of banana (Musa paradisiaca L.) var. Robusta. I.J.A.B.l.R,
4(2), Pp: 153-157.
15. Savangikar, V.A. 2002. Role of low cost options in tissue culture. In: Low cost options for
tissue culture technology in developing countries. Proc. of technical organized by the joint
FAO/IAEA division of nuclear techniques in food and agriculture, 2002, IAEA.
16. Sharma, S., Venkatasalam, EP., Patial, R., Latawa, J., and Singh, S. 2011. Influence of
gelling agents and nodes on the growth of potato microplant. Potato J. 38(1): 41-46.
17. Spooner, D.M., McLean, K., Ramsay, G., Waugh, R., and Bryan, G.J. 2005. A single
domestication for potato based on multilocus amplified fragment length polymorphism
genotyping. PNAS, 102: 14694–99.
18. Struik, P.C. 2007. Above-ground and below-ground plant development. In: D. Vreugdenhil
(ed), J. Bradshaw, C., Gebhardt, F., Govers, D.K.L., Mackerron, M.A., Taylor and H.A.,
Ross. Potato Biology and Biotechnology Advances and Perspectives. Elsevier Ltd, Oxford,
UK. 219-236.
19. Uranbey, S., Parmaksız, İ., Sancak, C., Çöçü, S., and Özcan, S. 2004. Temperature and
gelling agent effects on in vitro microtuberization of potato (Solanum tuberosum L.).
Biotechnol Biotec EQ, 18(2): 89-94.
20. Veramendi, J., Villafranca, M.J., Sota, V., and Mingo-Castel, A.M. 1997. Gelrite as an
alternative to agar for micropropagation and microtuberization of Solanum tuberosum L. cv.
Baraka. In Vitro Cellular Dev. Biol- Plant, 33: 195-199.
21. Wasserman, L. 2007. All of Nonparametric Statistics. Springer. 270p.