Waterlogging period duration and temperature effects on vegetative properties and glycolytic-fermentative enzymes activity in cotton seedling (Gossypium hirsutum L.) *

Waterlogging is one of the problems of cotton-growing in rainy regions which affects adversely plant growth in the early stages. So that rainfall causes waterlogging in early to mid spring in the fields without proper drainage. Also, rainfall declines soil and environment temperatures. To investigate the effects of waterlogging period duration and temperature on vegetative properties and glycolytic-fermentative enzymes activity in cotton, an experiment was carried out in laboratory and green house at Gorgan University of Agricultural Sciences and Natural Resources. Experimental treatments were the waterlogging period duration (0, 24, 48 and 96 h) and temperature (16, 20 and 25 ˚C). The results showed that increasing waterlogging period duration from 0 to 96 h and decreasing temperature from 25 to 16 ˚C led to decrease of leaf area, plant height and dry weight, chlorophyll and F_v/F_m ratio. However, the interaction of waterlogging period duration and temperature was significant only on leaf area, plant height and dry weight. The results showed that alcohol dehydrogenase and fructose-1,6-bisphosphate aldolase enzymes was affected by waterlogging period duration and temperature; but the interaction of waterlogging period duration and temperature was significant only on fructose-1,6-bisphosphate aldolase activity. So it can be concluded that in waterlogging conditions, decreasing temperature led to decrease cotton growth. Also, according to that alcohol dehydrogenate and fructose-1,6-bisphosphate aldolase enzymes activity show an increasing trend in waterlogging conditions, we can use from both enzymes as proper marker in order to determination of tolerant genotypes to waterlogging.