اثر محلول‌پاشی اسید آسکوربیک بر برخی صفات مورفولوژیک، فیزیولوژیک و عملکرد ذرت (SC704) در شرایط قطع آبیاری

نوع مقاله : مقاله کامل علمی- پژوهشی

نویسندگان

1 استاد، گروه زراعت، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران

2 دانشجوی دکتری، گروه زراعت، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران

3 دانش‌آموخته کارشناسی ارشد، گروه زراعت، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران

4 استادیار، گروه زراعت، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران

چکیده

سابقه و هدف: امروزه تنش خشکی یکی از بزرگ‌ترین تهدیدهایی است که در بسیاری از نقاط جهان بشر با آن مواجه است. تنش خشکی با عدم تعادل مواد غذایی و تولید گونه‌های فعال اکسیژن همراه است که منجر به کاهش رشد و عملکرد گیاهان زراعی می‌شود. بررسی‌ها نشان می‌دهد کاربرد خارجی اسید آسکوربیک به‌طور قابل‌توجهی اثرات بازدارنده تنش خشکی را بر رشد و متابولیسم گیاه بهبود می‌بخشد. با توجه به اهمیت تنش کم‌آبی این پژوهش جهت بررسی اثر اسید آسکوربیک بر برخی صفات دانه ذرت در شرایط زمان قطع آبیاری مورد ارزیابی قرار گرفت.
مواد و روش: این پژوهش در مزرعه آموزشی شماره یک دانشگاه علوم کشاورزی و منابع طبیعی گرگان در سال 1397 بر روی ذرت رقم سینگل کراس 704 (SC704) تحت شرایط تنش خشکی به صورت آزمایش اسپیلت پلات در قالب طرح بلوک‌های کامل تصادفی با 3 تکرار و 2 فاکتور انجام شد. تیمارهای آزمایش شامل: 1- تنش خشکی در 3 سطح (آبیاری کامل (بدون تنش)، و قطع آبیاری از مرحله شیری (تنش متوسط)، قطع آبیاری از مرحله ابریشم‌دهی (تنش شدید) 2- مقادیر مختلف اسید آسکوربیک در 7 سطح 0، 50، 100، 150، 200، 250 و 300 میلی‌گرم در لیتر بود. محلول‌پاشی در دو مرحله 6 برگی و ابریشم‌دهی انجام شد. در این پژوهش صفات، رنگیزه‌های فتوسنتزی شامل کلروفیلa، کلروفیل b در مرحله رویشی و زایشی، نشت الکترولیت، محتوای نسبی آب برگ، ارتفاع بوته، درصد پروتئین دانه، عملکرد زیستی و عملکرد دانه مورد بررسی و اندازه‌گیری قرار گرفت. تجزیه‌وتحلیل داده‌های حاصل از آزمایش با استفاده از نرم‌افزار SAS و آزمون LSD صورت گرفت.
یافته‌ها: نتایج نشان داد اثر متقابل کاربرد اسید آسکوربیک و زمان قطع آبیاری بر کلروفیل b در مرحله رویشی و زایشی، نشت الکترولیت، محتوای نسبی آب برگ، ارتفاع بوته، درصد پروتئین دانه، عملکرد زیستی و عملکـرد دانـه معنی‌دار شد. بیشترین عملکرد دانه در هنگام آبیاری کامل و قطع آبیاری از مرحله شیری در تیمار 150 میلی‌گرم در لیتر اسید آسکوربیک (به ترتیب برابر با 0/9678 و 1/9090 کیلوگرم در هکتار) بدست آمد و در هنگام قطع آبیاری از مرحله ابریشم دهی در کاربرد 300 میلی‌گرم در لیتر اسید آسکوربیک معادل 0/7395 مشاهده شد. در بررسی میزان کلروفیل، قطع آبیاری به تنهایی سبب کاهش این تیمار شد. همچنین نتایج بررسی میزان نشت الکترولیت و هدایت الکتریکی نشان داد کاربرد اسید آسکوربیک باعث افزایش در محتوای نسبی آب برگ و همچنین کاهش در نشت الکترولیت‌ها شد و محلول‌پاشی اسید آسکوربیک تا حدودی توانست اثرات مخرب ناشی از قطع آبیاری را کاهش دهد.
نتیجه‌گیری: نتایج نشان داد تنش خشکی سبب کاهش تیمارهای رنگیزه‌های فتوسنتزی، محتوای نسبی آب برگ، عملکرد دانه، عملکرد زیستی، تعداد دانه در بلال، درصد پروتئین دانه، ارتفاع بوته شد که محلول‌پاشی اسید آسکوربیک توانست تا حد زیادی از اثرات شدید و مخرب تنش جلوگیری کند. به ‌طورکلی بر اساس نتایج این آزمایش می‌توان از دز مناسب اسید آسکوربیک برای کاهش اثر تنش خشکی بر صفات ذکر شده و بخصوص عملکرد دانه و عملکرد زیستی استفاده کرد.
کلمات کلیدی: تنش خشکی، زمان قطع آبیاری، اسید آسکوربیک، کلروفیل، عملکرد دانه

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

The effect of ascorbic acid foliar spraying on some morphological, physiological and grain yield of corn (SC704) under irrigation cut-off conditions

نویسندگان [English]

  • Serollah Galeshi 1
  • Mitra Toloo hafezian aval 2
  • Seyyed Mansour Hosseini 3
  • Hosein Maleka 2
  • Benjamin Torabi 1
  • Mohammad hossein ghorbani 4
1 Professor, Department of Agronomy, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
2 Ph.D. candidate, Department of Agronomy, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
3 M.Sc Graduate Student, Department of Agronomy, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
4 Assistant Professor, Department of Agronomy, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
چکیده [English]

Background and purpose: Today, drought stress is one of the greatest threats facing humanity in many parts of the world. Drought stress is associated with nutrient imbalance and production of reactive oxygen species, which leads to reduced growth and yield of crop plants. Studies show that the external application of ascorbic acid significantly improves the inhibitory effects of drought stress on plant growth and metabolism. Given the importance of water deficit stress, this study was conducted to investigate the effect of ascorbic acid on some corn grain traits under irrigation-free conditions.
Materials and methods: This research was conducted in educational farm No. 1 of Gorgan University of Agricultural Sciences and Natural Resources in 2017 on Single Cross 704 (SC704) corn under drought stress conditions in the form of a split-plot experiment in the form of a randomized complete block design with 3 Repetition and 2 factors were done. The experimental treatments include: 1- drought stress at 3 levels (full irrigation (no stress), interruption of irrigation from the silking stage (severe stress) and interruption of irrigation from the milking stage (moderate stress) 2- different amounts of ascorbic acid at 7 levels 0, 50, 100, 150, 200, 250 and 300 mg/l. Spraying was done in two stages of 6 leaves and silking. In this research, the photosynthetic pigments include chlorophyll a and chlorophyll b in the vegetative stage and fertility, electrolyte leakage, relative leaf water content, plant height, grain protein percentage, number of grain per spike, and biological yield were measured. The data analysis was done using SAS and LSD test software
Results: In the study of chlorophyll content, irrigation cessation alone caused a decrease in this treatment. Also, the results of the study of electrolyte leakage and electrical conductivity showed that the use of ascorbic acid increased the relative leaf water content and also reduced the electrolyte leakage, and foliar application of ascorbic acid was able to reduce the destructive effects of irrigation cessation to some extent.
Conclusion: The results showed that drought stress caused a decrease in the treatments of photosynthetic pigments, relative leaf water content, grain yield, biological yield, number of grains per ear, grain protein percentage, and plant height, and foliar application of ascorbic acid was able to largely prevent the severe and destructive effects of stress. In general, based on the results of this experiment, an appropriate dose of ascorbic acid can be used to reduce the effect of drought stress on the mentioned traits, especially grain yield and biological yield.
Key words: Drought stress, Irrigation cut-off time, Ascorbic acid, Chlorophyll, Grain yield.

کلیدواژه‌ها [English]

  • Drought stress
  • Irrigation cut-off time
  • Ascorbic acid
  • Chlorophyll
  • Grain yield
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