نوع مقاله : مقاله کامل علمی- پژوهشی
نویسندگان
1 استاد، گروه زراعت، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران
2 دانشجوی دکتری، گروه زراعت، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران
3 دانشآموخته کارشناسی ارشد، گروه زراعت، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران
4 استادیار، گروه زراعت، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران
چکیده
کلیدواژهها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
Background and purpose: Today, drought stress is one of the greatest threats facing humanity in many parts of the world. Drought stress is associated with nutrient imbalance and production of reactive oxygen species, which leads to reduced growth and yield of crop plants. Studies show that the external application of ascorbic acid significantly improves the inhibitory effects of drought stress on plant growth and metabolism. Given the importance of water deficit stress, this study was conducted to investigate the effect of ascorbic acid on some corn grain traits under irrigation-free conditions.
Materials and methods: This research was conducted in educational farm No. 1 of Gorgan University of Agricultural Sciences and Natural Resources in 2017 on Single Cross 704 (SC704) corn under drought stress conditions in the form of a split-plot experiment in the form of a randomized complete block design with 3 Repetition and 2 factors were done. The experimental treatments include: 1- drought stress at 3 levels (full irrigation (no stress), interruption of irrigation from the silking stage (severe stress) and interruption of irrigation from the milking stage (moderate stress) 2- different amounts of ascorbic acid at 7 levels 0, 50, 100, 150, 200, 250 and 300 mg/l. Spraying was done in two stages of 6 leaves and silking. In this research, the photosynthetic pigments include chlorophyll a and chlorophyll b in the vegetative stage and fertility, electrolyte leakage, relative leaf water content, plant height, grain protein percentage, number of grain per spike, and biological yield were measured. The data analysis was done using SAS and LSD test software
Results: In the study of chlorophyll content, irrigation cessation alone caused a decrease in this treatment. Also, the results of the study of electrolyte leakage and electrical conductivity showed that the use of ascorbic acid increased the relative leaf water content and also reduced the electrolyte leakage, and foliar application of ascorbic acid was able to reduce the destructive effects of irrigation cessation to some extent.
Conclusion: The results showed that drought stress caused a decrease in the treatments of photosynthetic pigments, relative leaf water content, grain yield, biological yield, number of grains per ear, grain protein percentage, and plant height, and foliar application of ascorbic acid was able to largely prevent the severe and destructive effects of stress. In general, based on the results of this experiment, an appropriate dose of ascorbic acid can be used to reduce the effect of drought stress on the mentioned traits, especially grain yield and biological yield.
Key words: Drought stress, Irrigation cut-off time, Ascorbic acid, Chlorophyll, Grain yield.
کلیدواژهها [English]
1.Pal, B., & Jat, S. L. (2024). Enhancing state-wide corn (Zea mays L.) productivity by bridging the yield gap between top and average farmers in India. Maize Journal, 13(2), 1-5.
2.Bell, J. (2017). Corn Growth Stages and Development; Texas A&M AgriLife Extension and Research Agronomist, Amarillo: Lubbock, TX, USA.
3.Yadava, P., Abhishek, A., Singh, R., Singh, I., Kaul, T., Pattanayak, A., & Agrawal, P.K. (2017). Advances in maize transformation technologies and development of transgenic maize. Frontiers in Plant Science. 7:1949.
4.Adewale, S.A., Akinwale, R.O., Fakorede, M.A.B., & Badu-Apraku, B. (2018). Genetic analysis of drought-adaptive traits at seedling stage in early-maturing maize inbred lines and field performance under stress conditions. Euphytica, 214, 1–18.
5.Webber, H., Ewert, F., Olesen, J.E., Müller, C., Fronzek, S., Ruane, A.C., Martre, P., Ababaei, B., & Bindi, M. (2018). Diverging importance of drought stress for maize and winter wheat in Europe. Nature Communications, 9, 4249.
6.EPA. (2021). Available: https://www.epa.gov/climate-indicators/weather-climate (accessed January 25).
7.IPCC. (2013). “Policymakers,” in Climate Change 2013: The Physical Science Basis. Contribution of Working Group I to the Fifth Assessment Report of the Intergovernmental Panel on Climate Change, eds T. F. Stocker, D. Qin, G.-K. Plattner, M. Tignor, S. K. Allen, J. Boschung, et al. (Cambridge, MA: Cambridge University Press).
8.Pereira, L.F.M., Santos, H.L., Zanetti, S., de Oliveira Brito, I.A., dos Santos Tozin, L.R., Rodrigues, T.M., & de Almeida Silva, M. (2022). Morphology, biochemistry, and yield of cassava as functions of growth stage and water regime. South African Journal of Botany, 149, 222-239.
9.Haghpanah, M., Hashemipetroudi, S., Arzani, A., & Araniti, F. (2024). Drought Tolerance in Plants: Physiological and Molecular Responses. Plants, 13(21), 2962.
10.Galeshi, S. (2015). The effect of environmental stresses on plants (volume one) (drought, salinity, heat and waterlogging). Publications of Gorgan University of Agricultural Sciences and Natural Resources. 388pp.
11.Trachsel, S., Sun, D., SanVicente, F. M., Zheng, H., Atlin, G. N., Suarez, E. A., Babu, R., & Zhang, X. (2016). Identification of QTL for early vigor and stay-green conferring tolerance to drought in two connected advanced backcross populations in tropical maize (Zea mays L.). PLoS One 11: e0149636.
12.Sah, R.P., Chakraborty, M., Prasad, K., Pandit, M., Tudu, V.K., Chakravarty, M.K., Narayan, S.C., Rana, M. & Moharana, D. (2020). Impact of water deficit stress in maize: phenology and yield components. Scientific Reports. 10, 1–5.
13.Naz, H.I.R.A., Akram, N.A., & Ashraf, M. (2016). Impact of ascorbic acid on growth and some physiological attributes of cucumber (Cucumis sativus) plants under water-deficit conditions. Pakistan Journal of Botany, 48(3), 877-883.
14.Saleem, M.H., Ali, S., Rehman, M., Rana, M.S., Rizwan, M., Kamran, M., Imran, M., Riaz, M., Mona H. Soliman, M.H., Elkelish, A., & Liu, L. (2020). Influence of phosphorus on copper phytoextraction via modulating cellular organelles in two jute (Corchorus capsularis L.) varieties grown in a copper mining soil of Hubei Province, China. Chemosphere, 248.
15.Rezayian, M., Niknam, V., & Ebrahimzadeh, H. (2019). Oxidative damage and antioxidative system in algae. Toxicology reports, 6, 1309-1313.
16.Weng, M., Cui, L., Liu, F., Zhang, M., Yang, S.S., & Deng. X. (2015). Effects of drought stress on antioxidant enzymes in seedlings of different wheat genotypes. Pakistan Journal of Botany, 47(1), 49-56.
17.Shafiq, S., Akram, N.A., Ashraf, M., & Arshad, A. (2014). Synergistic effects of drought and ascorbic acid on growth, mineral nutrients and oxidative defense system in canola (Brassica napus L.) plants. Acta Physiologiae Plantarum, 36, 1539-1553
18.Yadollahi, P., Asgharipour, M.R., & Sheikhpour, S. (2015). Effects of ascorbic acid on growth and photosynthetic pigments of basil under arsenic toxicity. Journal of crop ecophysiology (agriculture science), 8, (32), 553-566. [in persian]
19.Akhlaghi, H., Mahdavi, B., & Rezaei, H. (2018). Characterization of chemical composition and antioxidant properties of Trachyspermum ammi seed as a potential medicinal plant. Journal of Chemical Health Risks, 4(4), 9-16.
20.Sharma, R., Bhardwaj, R., Thukral, A.K., Al-Huqail, A.A., Siddiqui, M.H., & Ahmad, P. (2019). Oxidative stress mitigation and initiation of antioxidant and osmoprotectant responses mediated by ascorbic acid in Brassica juncea L. subjected to copper (II) stress. Ecotoxicology and environmental safety, 109436.
21.Towhidi Moghaddam, H. (2017). Effect of foliar application of ascorbic acid on quantitative and qualitative traits as well as some biochemical changes in leaves of grain corn (Zea maize L.) under water deficit stress. Iranian Journal of Field Crop Science, 48(2), 365-375. [In Persian]
22- Asghari, M., Masoumi Zavariyan, A., & Yousefi rad, M. (2016). The effect of foliar application of ascorbic acid on yield components and physiologic character of sweet corn under different irrigation regims. Cereal Research, 6(2), 229-240. [In Persian]
23.Bilska, K., Wojciechowska, N., Alipour, S., & Kalemba, E.M. (2019). Ascorbic acid-The little-known antioxidant in woody plants. Antioxidants, 8(12), 645-651.
24.Darvishan, M., Tohidi-Moghadam, H.R., & Zahedi, H. (2013). The effect of foliar application of ascorbic acid (vitamin C) on physiological and biochemical changes of corn (Zea mays L.) under irrigation withholding in different growth stages. Maydica, 58, 195-200.
25.Noctor, G., & Foyer, C.H. (2018). Ascorbate and glutathione: Keeping active oxygen under control. Annual Review of Plant Physiology and Plant Molecular Biology, 49, 249-279.
26.Abdul Qados, A.M.S. (2014). Effect of ascorbic acid Antioxidant on soybean (Glycine max L.) planta grown under water stress conditions. International Journal of Advanced Research in Biological Sciences, 1(6), 189-205.
27.Hassan, A., Amjad, S.F., Saleem, M.H., Yasmin, H., Imran, M., Riaz, M., Qurban Ali, Q., Joyia, F.A., Mobeen. Ahmed, Sh., Ali, Sh, Abdullah Alsahli, A., & Alyemeni, M.N. (2021). Foliar application of ascorbic acid enhances salinity stress tolerance in barley (Hordeum vulgare L.) through modulation of morpho-physio-biochemical attributes, ions uptake, osmo-protectants and stress response genes expression. Saudi Journal of Biological Sciences, 28(8), 4276-4290.
28.Zhou, X., Gu, Z., Xu, H., Chen, L., Tao, G., Yu, Y., & Li, K. (2016). The effects of exogenous ascorbic acid on the mechanism of physiological and biochemical responses to nitrate uptake in two rice cultivars (Oryza sativa L.) under aluminum stress. Journal of Plant Growth Regulation, 35, 1013-1024.
29.Szepesi, A., Poor, P., Gemes, K., Horvath, E., & Tari, I. (2015). Influence of exogenous salicylic acid on antioxidant enzyme activities in the roots of salt stressed tomato plants. Acta Biologica Szegediensis, 52, 199-200.
31.Ardakani, M. & Nadur, A. (2009). Principles and Techniques for Plant Scientists. Tehran University Press. 270p.
32.Ritchie, S.W., & Nguyen, H.T. (1990). Leaf water content and gas exchange parameters of two wheat genotypes differing in drought resistance. Crop Science, 30, 105-111.
33.Lutts S., Kinet, J.M., & Bouharmont, J. (1996). NaCl induced senescence in leaves of rice (Oryza sativa) cultivars differing in salinity resistance. Annals of Botany, 78, 389-398.
34.Shuman, L.M., Boswell, F.C., Okhi, K., Parker, M.B., & Wilson. D.O. (1980). Critical soil manganese deficiency levels for four extractants for soybean grown in sandy soil. Soil Science Society of America Journal. 44, 1021-1025.
35.Soltani, E. (2007). Application of SAS software in statistical analyses. University Jahad of Mashhad. 182 p. [In Persian]
36.Hosseini, S.J., Tahmasebi‐Sarvestani, Z., Pirdashti, H., Modarres‐Sanavy, S.A.M., Mokhtassi‐Bidgoli, A., Hazrati, S., & Nicola, S. (2021). Investigation of yield, phytochemical composition, and photosynthetic pigments in different mint ecotypes under salinity stress. Food Science & Nutrition, 9(5), 2620-2643.
37.Huang, C.J., Wei, G., Jie, Y.C., Xu, J.J., Zhao, S.Y., Wang, L.C., & Anjum, S.A. (2015). Responses of gas exchange, chlorophyll synthesis and ROS-scavenging systems to salinity stress in two ramie (Boehmeria nivea L.) cultivars. Photosynthetica, 53 (3), 455–463.
38- Tanveer, M., Shahzad, B., Sharma, A., Biju, S., & Bhardwaj, R. (2018). 24-Epibrassinolide; an active brassinolide and its role in salt stress tolerance in plants: A review. Plant Physiolgy and Biochemistry. 130, 69–79.
39.Wu, Y., Jin, X., Liao, W., Hu, L., Dawuda, M.M., Zhao, X., Tang, Z., Gong, T., & Yu, J. (2018). 5-Aminolevulinic acid (ALA) alleviated salinity stress in cucumber seedlings by enhancing chlorophyll synthesis pathway. Frontiers in Plant Science, 9, 635-642.
40.Azeem, M., Sultana, R., Mahmood, A., Qasim, M., Siddiqui, Z.S., Mumtaz, S., Javed, T., Umar, M., Adnan, M.Y., & Siddiqui, M.H. (2023). Ascorbic and salicylic acids vitalized growth, biochemical responses, antioxidant enzymes, photosynthetic efficiency, and ionic regulation to alleviate salinity stress in sorghum bicolor. Journal of Plant Growth Regulation, 42(8), 5266-5279.
41- Mahdavifard, M., Abdolhossein Rezaeinejad, A.H., & Mosavifard, S. (2020). Effect of light intensity and ascorbic acid on some morphological and physiological characteristics of Zinnia elegans L. Journal of Plant Research, 32(4), 784-791 [In Persian]
42- Farjam, S., Siosemardeh, A., Kazemi-Arbat, H., Yarnia, M., & Rokhzadi, A. (2014). Response of chickpea (Cicer arietinum L.) to exogenous salicylic acid and ascorbic acid under vegetative and reproductive drought stress conditions. Journal of Applied Botany and Food Quality, 87, 80-86.
43.Shemi, R., Wang, R., Gheith, E.S.M., Hussain, H.A., Hussain, S., Irfan, M., Cholidah, L., Zhang, K., Zhang, S., & Wang, L. (2021). Effects of salicylic acid, zinc and glycine betaine on morpho-physiological growth and yield of maize under drought stress. Scientific Reports, 11(1), 3195.
44.Moharramnejad, S., Sofalian, O., Valizadeh, M., Asghari, A., Shiri, M.R., & Ashraf, M. (2019). Response of maize to field drought stress: oxidative defense system, osmolytes’ accumulation and photosynthetic pigments. Pakistan Journal of Botany, 51, 799–807.
45.Saha, S., Begum, H. H., Nasrin, S., & Samad, R. (2020). Effects of drought stress on pigment and protein contents and antioxidant enzyme activities in five varieties of rice (Oryza sativa L.). Bangladesh Journal of Botany, 49(4), 997-1002.
46.Maqbool, M.M., Ali, A., Haq, T., Majeed, M.N., & Lee, D.J. (2015). Response of spring wheat (Triticum aestivum L.) to induced water stress at critical growth stages. Sarhad Journal of Agriculture, 31(1), 53-58.
47.Sohag, A.A. M., Tahjib-Ul-Arif, M., Brestic, M., Afrin, S., Sakil, M.A., Hossain, M.T., & Hossain, M.A. (2020). Exogenous salicylic acid and hydrogen peroxide attenuate drought stress in rice. Plant Soil Environment, 66(1), 7-13
48 Naik, B. V., Ahmad, M. A., Jangde, S., Sanodiya, P., Ray, M., Reddy, K. M., & Jat, M. (2024). Effects of Foliar Application of Salicylic Acid On Morphological, Biochemical and Yield Attributes of Maize (Zea Mays L.) Under Rainfed Condition. Bangladesh Journal of Botany, 53(2), 273-278.
49.Sofy, M.R., Seleiman, M.F., Alhammad, B.A., Alharbi, B.M., & Mohamed, H.I. (2020). Minimizing adverse effects of pb on maize plants by combined treatment with jasmonic, salicylic acids and proline. Agronomy, 10(5), p.699.
50- Pawar, K.R., Wagh, S.G., Sonune, P.P., Solunke, S.R., Solanke, S.B., Rathod, S.G., & Harke, S.N. (2020). Analysis of water stress in different varieties of maize (Zea mays L.) at the early seedling stage. Biotechnology Journal International, 24(1), 15-24.
51- Qanbari theylami, N., Abbaspour, H., & Baradaran Firouzabadi, M. (2014). The effect of ascorbic acid and methanol foliar spraying on dry matter accumulation and yield of DPX soybean under water deficit conditions. Scientific-research journal of plant ecophysiology, 6(17), 13-27. [In Persian]
52- Baradaran Firouzabadi, M., Parsaeiyan, M., & Baradaran Firouzabadi. M. (2017). Agronomic and physiological response of Nigella sativa L. to ascorbate and methanol foliar application in water deficit stress. Plant ecophysiology (arsanjan branch), 9(30): 13-27. [In Persian]
53- Kalantar Ahmadi, S.A., Ebadi, A., Daneshian, J., Siadat, S.A., & Jahanbakhsh, S. (2016). Effect of drought stress and foliar application of growth regulators on photosynthetic pigments and seed yield of rapeseed (Brassica napus L. Cv. Hyola 401). Iranian journal of crop sciences, 18(3), 196-217. [In Persian]
54- Kaman, H., Kirda, C., & Sesveren, S. (2015). Genotypic differences of maize in grain yield response to deficit irrigation. Agricultural Water Management, 98(5): 801-807.
55- Sharma, L., Roy, S., Satya, P., Alam, N.M., Goswami, T., Barman, D., Bera, A., Saha, R., Mitra, S., & Mitra, J. (2024). Exogenous ascorbic acid application ameliorates drought stress through improvement in morpho-physiology, nutrient dynamics, stress metabolite production and antioxidant activities recovering cellulosic fibre production in jute (Corchorus olitorius L.). Industrial Crops and Products, 217, 118808.
56- Azarpour, A. (2016). The effect of methanol and vitamin C foliar application on the growth and yield of peanut (Arachis hypogeac) under rainfed conditions. Ph.D. Thesis. Gilan University. 117 p. [In Persian]