نوع مقاله : مقاله کامل علمی- پژوهشی
نویسندگان
1 دکتری اصلاح نباتات، دانشگاه علوم کشاورزی و منابع طبیعی ساری،
2 استاد، گروه اصلاح نباتات، دانشگاه علوم کشاورزی و منابع طبیعی ساری،
3 دانشیار، گروه اصلاح نباتات، دانشگاه علوم کشاورزی و منابع طبیعی ساری
4 استاد، گروه مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی، دانشگاه لرستان،
چکیده
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
Background and Objectives: Salinity stress is a very serious threat to most agricultural products in the world. In the world, rice ranks second after wheat and is a relatively sensitive plant to salt stress. Protein kinases are an important group of kinase enzymes that phosphorylate target proteins by adding phosphate groups. These enzymes play an important role in cell communication by inducing the message of growth and reproduction. Gene expression is a process during which a useful gene product is synthesized by the information obtained from a gene. The purpose of this research was to investigate the expression level of SAPK1 gene from the protein kinase gene group in rice plants under sodium chloride salt stress by examining three different times after the stress was applied
Materials and methods: In this study, the expression pattern of SAPK1 gene in two Cultivars tolerant (Shastak Mohammadi) and sensitive (IR29) of rice plant under salt stress was investigated by factorial experiment in the form of a completely randomized design with three replications. Cultivars were planted in the research greenhouse of the Faculty of Agriculture of Lorestan University and at the seedling stage (5 to 6 leaves), salinity stress was applied to the plant at three levels of 3, 6, and 9 dS/m and the control treatment (without salt stress). Then, at three different times (6, 12, and 24 hours after applying stress), plant leaves were sampled to investigate gene expression. The leaf samples were stored in a freezer at minus 80 degrees Celsius. Then, RNA extraction and cDNA synthesis were done by special kits for each stage, and finally,
Results: The results of the analysis of SAPK1 gene expression pattern showed that in both tolerant and sensitive cultivars, the highest level of gene expression was observed at the salinity level of 9 ds/m 24 hours after applying the stress. At different times after applying the stress in the control treatment and the salinity level of 3 ds/m, the gene expression level in both tolerant and sensitive cultivars did not show any difference and was very insignificant. However, at the salinity level of 6 ds/m, 6 hours after applying the stress, the gene expression level in the tolerant cultivar increased 3 fold compared to the sensitive cultivar and control treatment. At this level, examining gene expression 12 and 24 hours after applying the stress, the amount of gene expression in the tolerant cultivar was about 7 fold higher than the sensitive cultivar and the control treatment. At the salinity level of 9 ds/m, 6 hours after applying the stress, the gene expression level in the tolerant cultivar was doubled compared to the sensitive cultivar and 4 fold higher than the control treatment. While 12 hours after applying the stress, the gene expression level in the tolerant cultivar increased by 5.5 fold compared to the sensitive cultivar and by 10 fold compared to the control treatment. Also, 24 hours after applying the stress, gene expression in tolerant cultivar was 3.5 fold higher than the sensitive cultivar and 11 fold higher than the control treatment.
Conclusion: These results showed that the expression of SAPK1 gene increases in rice plants with increasing salinity levels. nvestigating the effect of time after applying stress on SAPK1 gene expression, it was found that in the early hours after applying stress, the amount of gene expression was low, but with increasing time up to 24 hours, gene expression in tolerant cultivar increased These results can be used in molecular studies of rice cultivars to select the most tolerant rice cultivars to salinity stress for cultivation in areas prone to salinity.
کلیدواژه‌ها [English]
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